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In this study, expressions of cell-cycle-related genes: Expressions of housekeeping genes such as beta-actin, cyclophilin and proliferating cell nuclear antigens PCNA are determined using reverse transcription polymerase chain reaction RT-PCR for the correction of unequal amount of cDNA added into the samples. Using labeled 32P-dCTP and Phosphorimager technology, relative abundance of radioactivities of the PCR products is obtained by dividing the radioactivity of each individual sample by the corresponding radioactivities of different housekeeping genes.
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