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To browse Academia.Screwing store Mix identified with air carbon dating archaeology Russian When humans consume foods from different radiocarbon reservoirs offset in age to the atmosphere, inaccuracies in the 14C date of bone collagen can occur. This has been demonstrated through direct dating of bulk collagen from human bones and the remains of ungulate bone projectile points that were found embedded in them Cook et al. We present improvements to a novel HPLC method for the detection and separation of underivatized amino acids using a water-only mobile phase free of organic or inorganic modifiers, ensuring very low carbon backgrounds. Our hypothesis is that direct 14C dating of single essential and non-essential amino acids might allow an improvement in the dating accuracy for reservoir-affected human bones. We discuss methodological developments, demonstrate carbon backgrounds, and present analytical approaches to minimize their effects.